With the normalization of COVID-19 prevention and control, PCR has become a part of people's daily life. Accurate and rapid identification of infected person is the key to epidemic prevention and control. Isolation and intervention of high-risk populations through early diagnosis of viruses is one of the most effective prevention and control measures at this stage.
All organisms except prions contain nucleic acids, which include deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Novel coronavirus (2019 nCoV) belongs to β Coronavirus genus. What makes COVID-19 invisible? At present, the most commonly used method is real-time fluorescent quantitative RT-PCR. By adding fluorescent groups in the PCR reaction system, and then using the fluorescence signal accumulation to conduct real-time detection, you can determine whether the sample is negative or positive according to the strength of the fluorescence signal.
After biochemical staining, nucleic acid test samples can be detected by flow cytometry. Flow cytometry is mainly composed of liquid flow system, optical system, detection system and analysis system. The fluid flow system is mainly used to form sheath fluid - single cell suspension; The optical system is mainly used to shape and modulate the laser beam into a beam suitable for detection, and collect the fluorescence signal excited by the laser; The detection system is mainly used for photoelectric conversion of the collected fluorescence signal; The analysis system mainly calculates and analyzes the amplified electrical signals, and finally gives the results.
All roads lead to Rome ? Or thousands of troops cross the single wooden bridge ?
Flowcell is the heart of the fluid flow system. The stained cells to be measured is lined up in the flowcell, and then is irradiated by laser one by one. These stained nucleic acid sample cells generate fluorescence signals through laser radiation excitation; The intensity of these fluorescent signals reflects the expression content of cell antigens. By analyzing the fluorescent signals, we can identify viruses.
Optic bonding is the core technology for processing flowcell. Optic bonding technology is a low-temperature molecular bonding technology that does not use any adhesive. It uses the force between molecules to achieve the bonding between lenses. It has the characteristics of high strength, strong damage threshold, zero pollution, etc. This will enable thousands of troops to cross the single wooden bridge!
4. Flowcell of FOCtek
In combination with its own professional high-precision cold processing technology, high-precision optic bonding technology and a variety of coating technologies, the flowcell developed by FOCtekt has the following characteristics, which can meet the request of medical application of detection and help PCR !
① High strength: no penetration under 15 psi gauge pressure;
② Ultra fine runner: 0.1mm * 0.2mm~* 0.4mm square runner, edge collapse<0.05mm;
③ Super clean runner: the finish in the runner S/D=0/0;
④ Centration of taper hole and runner ＜ 0.015mm;
⑤ Various coating .